Dev130344 15..23
نویسندگان
چکیده
To facilitate understanding the mechanisms of somatic reprogramming to human induced pluripotent stem cells (iPSCs), we have established intermediately reprogrammed stem cells (iRSCs), human mesenchymal cells that express exogenous Oct4, Sox2, Klf4 and c-Myc (OSKM) and endogenous SOX2 and NANOG. iRSCs can be stably maintained at low density. At high density, however, they are induced to enter mesenchymal-epithelial transition (MET), resulting in reprogramming to an iPSC state. Morphological changes through MET correlate with silencing of exogenous OSKM, and upregulation of endogenous OCT4. A CRISPR/Cas9-mediated GFP knock-in visualized the temporal regulation of endogenous OCT4 in cells converting from iRSC to iPSC state. OCT4 activation coincident with silencing of OSKM occurred prior to entering MET. Notably, OCT4 instability was frequently observed in cells of developing post-MET colonies until a late stage (>200 cells), demonstrating that OCT4-activated post-MET cells switched from asymmetric to symmetric cell division in late stage reprogramming.
منابع مشابه
Index of /econ/workingpapers
2094.html 09-Aug-00 10:32 1k bgr_99.pdf 20-Jun-00 11:31 1.0M double burden nov98.pdf17-Feb-99 14:34 200k foutax28-8.pdf 12-Sep-00 09:41 388k instoke.gif 22-Oct-99 13:34 7k macro10.pdf 14-Feb-00 13:16 313k merger0205.pdf 18-Sep-00 10:30 310k sok141.html 28-May-98 14:12 4k supermodular.pdf 14-Feb-00 13:16 164k workingpapers.html 22-Oct-99 13:24 21k wp0002.pdf 09-Jun-00 10:10 194k wp0004.pdf 09-Ju...
متن کاملA Bibliography of Publications in ACM SIGPLAN Notices, 1960–1969
+ [21]. 68 [23]. ALGOL [10, 15, 17, 18, 21, 23, 5, 20]. Algol-based [20]. ALGOL-N [21]. based [20]. Bulletin [24, 5, 10, 15, 17, 18, 12, 14, 4, 7, 11, 3, 6, 9, 16]. collateral [20]. considered [1]. defining [20]. execution [20]. FORTRAN [14, 12]. general [20]. generation [23]. Go [1, 2]. harmful [1]. hybrid [20]. infinite [22, 23]. information [12, 14, 4]. involving [23]. Jovial [4]. language [...
متن کاملPeer interaction in child care centres at 15 and 23 months: stability and links with children's socio-emotional adjustment.
In this longitudinal study, 70 children were observed during 90 min of free play in their child care centres at 15 and 23 months of age. The children (39 boys and 31 girls) attended 51 different care groups in 39 centres. The occurrence and stability of peer interactions in the second year of life and their relations to children's socio-emotional adjustment were examined. The frequency of child...
متن کاملAssessment of Exposure for Pesticide Handlers in Agricultural, Residential and Institutional Environments
INTRODUCTION 14 PESTICIDE CATEGORIES 15 PESTICIDE HANDLERS 15 Agricultural Pesticide Handlers 15 Tasks Performed by an Individual 16 Factors Affecting Exposure 16 Residential and Institutional Pesticide Handlers 18 Families of Pesticide Handlers 19 STUDY DESIGN CONSIDERATIONS 20 Worker Stratification 21 Routes of Exposure 21 Respiratory Exposure 21 Dermal Exposure 21 Sampling Strategy Selection...
متن کاملCarbapenemase-producing Bacteria in Patients Hospitalized Abroad, France
Technical Appendix Table. β-lactamase profiles of multidrug-resistant Gram-negative bacilli isolated in 2010 and 2011, France* Region of origin No. culture-positive patients/total no. patients Species No. isolates β-lactamase ESBL Carbapenemase pCASE Africa 33/87 E. coli 18 CTX-M-15 2 CTX-M-1 2 SHV-12 1 CTX-M-3 OXA-48 1 CTX-M-14 1 CTX-M-15 TEM-169 1 CTX-M-15 CMY-2 1 CTX-M-24 OXA-48 1 ND K. pneu...
متن کامل